小鼠骨骼肌细胞核DNA降解与死亡时间的关系

目的　监测小鼠死后骨骼肌核内DNA降解的情况 ,探讨死后细胞核DNA降解的一般规律。方法　建立小鼠死亡模型 ,在死后 72h内 ,以 12h的间隔取骨骼肌样本进行单细胞凝胶电泳 ,在荧光显微镜下测量彗星图像 ,并作统计学分析。结果　机体死后 ,骨骼肌细胞在电泳图像上出现了明显的彗星形拖尾 ,L/W比值随死亡时间而逐渐增大 ,二者呈一定的线性关系。结论　单细胞凝胶电泳技术可以应用于早期死亡时间推断。     

自动消色笔书写笔迹显现方法的实验研究

目的为自动消色笔书写笔迹的显现识别提供最佳方法。方法通过观察不同种类的自动消色笔书写在不同纸张上,保存在不同环境下的笔迹的消色过程、消色时间,分析判断消色笔书写笔迹的基本特性。使用文检仪、静电压痕仪、不同浓度的氢氧化钠溶液显现消色笔迹,比较不同显现识别方法的特点及优劣。结果不同的显现方法各有优劣,使用氢氧化钠溶液显色效果的最佳浓度为1.0 mol。结论在自动消色笔书写笔迹的显现中,可以根据不同情况,选择合适的显现方法达到识别检验的目的。     

签字笔水、圆珠笔油红外发光特性的研究

目的记录不同激发光、接收滤光镜下,各种油墨红外发光强弱的程度,为文字检验提供基础性的研究。方法用多波段光源中510~650nm单色光激发各种油墨样本,全波段物证检验CCD系统记录样本在530~950nm接收滤光镜下出现的红外发光图像。结果多数油墨在接收滤光镜到达800~850nm时,呈现出强烈的红外发光状态,并随接收波段的上升而开始衰减。结论可利用某些油墨的发光与否,发光强与弱的特性,检验案件中涂改和涂抹字迹。     

毒鼠强诱导细胞DNA损伤的彗星电泳检测

目的　研究毒鼠强对小鼠淋巴细胞和脑细胞DNA的损伤作用。方法　分离健康小鼠的淋巴细胞和脑细胞 ,以彗星电泳的方法测定不同浓度毒鼠强处理后的细胞DNA损伤。结果　 1/2 0～ 1/2LD50 剂量组的毒鼠强均可引起淋巴细胞和脑细胞不同程度的DNA损伤 ,与对照组呈极显著性差异 (P <0 0 0 1)。结论　毒鼠强引起细胞DNA断裂损伤 ,并呈现明显的剂量 -效应关系。     

Color Separation in Forensic Image Processing Using Interactive Differential Evolution

Color separation is an image processing technique that has often been used in forensic applications to differentiate among variant colors and to remove unwanted image interference. This process can reveal important information such as covered text or fingerprints in forensic investigation procedures. However, several limitations prevent users from selecting the appropriate parameters pertaining to the desired and undesired colors. This study proposes the hybridization of an interactive differential evolution (IDE) and a color separation technique that no longer requires users to guess required control parameters. The IDE algorithm optimizes these parameters in an interactive manner by utilizing human visual judgment to uncover desired objects. A comprehensive experimental verification has been conducted on various sample test images, including heavily obscured texts, texts with subtle color variations, and fingerprint smudges. The advantage of IDE is apparent as it effectively optimizes the color separation parameters at a level indiscernible to the naked eyes.     

Color separation in forensic image processing

In forensic image processing, it is often important to be able to separate a feature from an interfering background or foreground, or to demonstrate colors within an image to be different from each other. In this study, a color deconvolution algorithm that could accomplish this task is described, and it is applied to color separation problems in document and fingerprint examination. Subtle color differences (sometimes invisible to the naked eye) are found to be sufficient, which is demonstrated successfully for several cases where color differences were shown to exist, or where colors were removed from the foreground or background. The software is available for free in the form of an Adobe Photoshop-compatible plug-in.     

An evaluation of matching unknown writing inks with the United States International Ink Library

Utilizing a database of standards for forensic casework is a valuable resource. Undoubtedly, as more standards (and corresponding information about the specimens) are collected, there is a greater certainty of identification when a questioned and a known item cannot be distinguished after a series of analyses. The United States Secret Service and the Internal Revenue Service National Forensic Laboratory jointly maintain the largest known forensic collection of writing inks in the world, which is comprised of over 8500 ink standards collected worldwide, dating back to the 1920s. This study was conducted to evaluate the reliability of matching arbitrarily purchased pens with known inks from a database. One hundred pens were randomly obtained from a variety of sources and their respective ink compositions were compared with standards. Eighty-five of the inks were determined to be suitable for comparison utilizing optical examinations and thin-layer chromatography. Three of the inks did not match any of the specimens on record; one of these inks was similar to an ink from an identical brand of pen that was in the database, but had a modified formulation.     

A simple microthermal desorption device

A new method for thermal desorption of small samples is presented. The method uses a solid phase microextraction (SPME) holder with the fiber removed. The sample-for example, an ink sample on paper-is simply placed inside the needle of the holder, where normally an SPME fiber is positioned. The thermal desorption is then performed on any kind of gas chromatograph in a manner similar to that for SPME analysis. The needle of the SPME holder penetrates the injector septum; the temperature of the thermal desorption is simply the temperature of the injector. No solvents or liquid nitrogen cooling are used. The paper sample is kept inside the holder needle during the analysis. After the analysis is completed, the sample is removed from the needle by pushing forward the steel wire inside the needle in the way normally used to perform sampling with the SPME fiber. The desorbed compounds were analyzed by gas chromatography with a flame ionization detector or by gas chromatography-mass spectrometry. The optimum temperature for desorption of ink samples on paper was 200 degrees C. The influence of the paper matrix is negligible at that temperature. Laboratories lacking the commercial device for thermal desorption can use this cheap device for the analysis of, for example, writing ink, printing ink, and inkjet ink samples on paper. Other types of samples can be investigated but the size of samples suitable for analysis is limited.     

灌服毒鼠强诱导大鼠细胞DNA的损伤研究

目的研究毒鼠强体内染毒后,毒鼠强对大鼠脑细胞、心肌细胞、淋巴细胞DNA的损伤作用。方法选择健康Sprague-Dawley大白鼠20只,分成5组,每组4只,采用灌胃方法使大鼠毒鼠强体内染毒,按0.2,0.1,0.05,0.01mg·kg-1制作大鼠毒鼠强中毒模型,并以灌服生理盐水的健康大鼠为对照,分离实验大鼠的淋巴细胞、心肌细胞和脑细胞,用彗星电泳的方法测定不同浓度毒鼠强中毒后的细胞DNA损伤。结果0.2,0.1,0.05,0.01mg·kg-1剂量组的毒鼠强均可引起大鼠的淋巴细胞、心肌细胞和脑细胞DNA损伤,均与对照组差异有显著性(P<0.01)。结论毒鼠强诱导体内细胞DNA损伤可能是毒鼠强毒性作用机制之一。     

FTIR图像—单点模式法无损鉴别不同品牌激光打印机墨粉色痕

利用傅里叶红外图像系统,采用单点模式对收集到的八种品牌激光打印机墨粉色痕进行红外扫描,采集样品红外光谱,并对其中两种样品红外光谱进行二阶导数运算,得到对应的二阶导数光谱。实验表明,该方法可有效区分不同品牌激光打印机墨粉色痕,并可为其他书写材料无损检验鉴定提供借鉴。